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immunomagnetic beads  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec immunomagnetic beads
    Immunomagnetic Beads, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 110 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd133+immunomagnetic+beads/CD133+MicroBead+Kit%2C+human+-+lyophilized/pmc12860264-131-11-14
    Average 95 stars, based on 110 article reviews
    immunomagnetic beads - by Bioz Stars, 2026-09
    95/100 stars

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    Isolation:

    Article Title: LAPTM4B enhances the stemness of CD133 + liver cancer stem-like cells via WNT/β-catenin signaling
    Article Snippet: Isolation of CD133+ liver cancer stem-like cells (CSLCs) To obtain higher purity CD133+ CSLCs, we digested adherent cells (Huh7 and HepG2 cell lines) (Trypsin-0.25%EDTA, Thermo, 37°C, 2 minutes) and filtered them through a 30 μm sieve to prepare a single-cell suspension. .. CD133+ cells were isolated using CD133 immunomagnetic beads (Miltenyi Biotec). .. These cells were cultivated in ultra-low adsorption culture dishes with serum-free stem cell conditioned medium containing DMEM/F12, penicillin (100 IU/ml, Sigma, USA), streptomycin (100 μg/ml, Sigma, USA), basic fibroblast growth factor (bFGF, 20 ng/ml, Peprotech, USA), and epidermal growth factor (EGF, 20 ng/ml, Peprotech, USA).

    Article Title: LAPTM4B enhances the stemness of CD133 + liver cancer stem-like cells via WNT/β-catenin signaling
    Article Snippet: Isolation of CD133+ liver cancer stem-like cells (CSLCs) To obtain higher purity CD133+ CSLCs, we digested adherent cells (Huh7 and HepG2 cell lines) (Trypsin-0.25%EDTA, Thermo, 37°C, 2 minutes) and filtered them through a 30 μm sieve to prepare a single-cell suspension. .. CD133+ cells were isolated using CD133 immunomagnetic beads (Miltenyi Biotec). .. These cells were cultivated in ultra-low adsorption culture dishes with serum-free stem cell conditioned medium containing DMEM/F12, penicillin (100 IU/ml, Sigma, 4 USA), streptomycin (100 μg/ml, Sigma, USA), basic fibroblast growth factor (bFGF, 20 ng/ml, Peprotech, USA), and epidermal growth factor (EGF, 20 ng/ml,

    Blocking Assay:

    Article Title: Differential expression profile analysis of DNA damage repair genes in CD133 + /CD133 − colorectal cancer cells
    Article Snippet: Briefly, the single cell suspension was centrifuged at 800 × g for 10 min at room temperature, washed 3 times with PBS and resuspended in 300 μl isolation buffer (Miltenyi Biotec Inc., Shanghai, China). .. Subsequently, 100 μl FcR blocking reagent and 100 μl CD133 immunomagnetic beads (Miltenyi Biotec Inc., Shanghai, China) were added. ..

    Incubation:

    Article Title: Anticancer effects of curcumin on nude mice bearing lung cancer A549 cell subsets SP and NSP cells
    Article Snippet: .. Briefly, A549 cells were incubated with CD133 immunomagnetic beads (cat. no. 130-100-857; Miltenyi Biotec, Inc., Cambridge, MA, USA) for 30 min at 4°C. ..

    Article Title: Anticancer effects of curcumin on nude mice bearing lung cancer A549 cell subsets SP and NSP cells.
    Article Snippet: .. Briefly, A549 cells were incubated with CD133 immunomagnetic beads (cat. no. 130-100-857; Miltenyi Biotec, Inc., Cambridge, MA, USA) for 30 min at 4 ̊C. ..



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